Protective Effect of Quercetin on Endothelial Cell Injured by HO

SUN Jing; CHENG Jia-yi; TENG Dan; ZHANG Ying; CHEN Wen-na

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Protective Effect of Quercetin on Endothelial Cell Injured by HO

更新时间：2024-01-04

- Protective Effect of Quercetin on Endothelial Cell Injured by HO
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 5, Pages: 174-176(2012)
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- Published：2012
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SUN Jing, CHENG Jia-yi, TENG Dan, et al. Protective Effect of Quercetin on Endothelial Cell Injured by HO[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(5): 174-176.
DOI：

SUN Jing, CHENG Jia-yi, TENG Dan, et al. Protective Effect of Quercetin on Endothelial Cell Injured by HO[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(5): 174-176. DOI：

摘要

目的: 探讨槲皮素对血管内皮细胞损伤的保护作用及机制。方法: 采用体外培养的人脐静脉内皮细胞株ECV304

观察内皮细胞加入槲皮素8

2 mg·L-1培养24 h后

再加入H2O2培养18 h后

造成血管内皮细胞损伤模型。用流式细胞仪测定内皮细胞死亡率

用ELISA法检测内皮细胞培养液血栓调节蛋白(TM)的含量;荧光分光光度法测定内皮细胞培养液中乳酸脱氢酶(LDH)的活性。结果: 与H2O2损伤后的模型组相比

槲皮素可以减低细胞死亡率

[模型组细胞死亡率为(28.36±0.10)%

槲皮素高、中、低剂量分别为(8.34±0.01)%

(16.58±0.04)%

(10.12±0.02)%]

可使培养液中TM蛋白含量减少

[模型组为(58.5±18.8)μg·L-1

槲皮素高、中、低剂量为(32.7±9.7)

(27.8±1.9)

(32.1±8.6) μg·L-1]和LDH活性减少[模型组为(1 931.9±159.5)U·L-1

槲皮素高、中、低剂量为(1.092±126.2)

(1 159.6±274.2)

(1 223.5±120.5) U·L-1]。结论: 槲皮素能明显保护过氧化氢对内皮细胞的损伤

其保护作用与抗脂质过氧化、保护细胞的完整性有关。

Abstract

Objective: To evaluate the effects of quercetin on the injured vascular endothelial cells and its mechanism. Method: Endothelial cell culture was in vitro. The endothelial cell injury model was established by cultured with H2O2 for 18 h

Quercetin at dose of 8

2 mg·L-1 was cultured with the cells before the model establishment. Flow cytometry was used for determining endothelial cell death rate. Endothelial cell was detected by ELISA for medium thrombomodulin(TM) content; spectrophotometric determination was used for the culture medium lactate dehydrogenase(LDH) activity. Result: Compared to the model group

quercetin can lower endothelial cell death rate

reduce the content of TM and activity of LDH in the culture medium. Conclusion: Quercetin can obviously protect endothelial cells

and its protective effect is related to against lipid peroxidation

protection of the cell membrane.

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