Objective: To study the effect of the herbs for removing heat from blood to arrest bleeding in Boye decoction on thromboxane B2(TXB2) and 6-keto-prostaglandin F1 alpha(6-keto-PGF1α) in rats with bleeding caused by cold of insufficiency of spleen-stomach. Method: The SD rats were randomly divided into 4 groups: normal control

model

Boye decoction and herbs for removing heat from blood to arrest bleeding in Boye decoction. The model of cold of insufficiency of spleen-stomach was made by intemperance of taking food for 14 days. Interventions started from the 8th day of modeling

the control group and the model group were ig given normal saline. Boye decoction group was given the decoction at a dose of 9 g·kg-1 daily. The group of herbs for removing heat from blood to arrest bleeding in Boye decoction was given at 6 g·kg-1 daily. All the interventions were for 7 days. The effect on TXB2 and 6-keto-PGF1α in SD rats was observed by using enzyme linked immunosorbent assay. Result: Boye decoction could significantly increase the level of TXB2 and decrease the level of 6-keto-PGF1α

compared with model group and the group of herbs for removing heat from blood to arrest bleeding in Boye decoction. The group of herbs for removing heat from blood to arrest bleeding in Boye decoction did not show significant difference compared with the model control. Conclusion: The main hemostasis mechanism of the herbs for removing heat from blood to arrest bleeding in Boye decoction is likely related the regulation of TXB2 and 6-keto-PGF1α.