Purification Technology of Extracts from  and  in Shuanghuanglian by AB-8 Macroporous Resin

LI Shu-fang; WANG Shu-mei; LIANG Sheng-wang; WANG Zhan-hong

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Purification Technology of Extracts from and in Shuanghuanglian by AB-8 Macroporous Resin

更新时间：2024-01-04

- Purification Technology of Extracts from and in Shuanghuanglian by AB-8 Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 2, Pages: 4-7(2012)
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- Published：2012
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LI Shu-fang, WANG Shu-mei, LIANG Sheng-wang, et al. Purification Technology of Extracts from and in Shuanghuanglian by AB-8 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 4-7.
DOI：

LI Shu-fang, WANG Shu-mei, LIANG Sheng-wang, et al. Purification Technology of Extracts from and in Shuanghuanglian by AB-8 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 4-7. DOI：

摘要

目的: 优化AB-8大孔吸附树脂纯化双黄连中金银花、连翘提取物的工艺。方法: 以提取物中总酚、总黄酮的含量为考察指标

从树脂径高比、吸附浓度、吸附流速、洗脱剂浓度等方面对大孔吸附树脂纯化工艺进行优化。结果: 最佳纯化工艺为金银花、连翘提取物

加蒸馏水稀释成1.5 g·mL-1(生药量)

树脂柱径高比1∶10

最大吸附量与树脂体积比为1∶3.3

水洗3倍树脂体积

4BV 70%乙醇洗脱。结论: AB-8大孔吸附树脂纯化金银花、连翘提取物的最佳工艺稳定可行。

Abstract

Objective: To optimize purification technology of extracts from L. japonicae and F. suspensa in Shuanghuanglian by AB-8 macroporous resin. Method: The content of total phenolic and total flavonoids were used as indexes.Purification technology was optimized from proportion of diameter-height

adsorption concentration

adsorption speed and eluent concentration

etc. Result: Optimum purification technology was as follows: extracts of L. japonicae and F. suspensa were diluted to concentration was 1.5 g·mL-1by water

ratio of diameter-height 1∶10

proportion of maximum adsorption to resin volume was 1∶3.3

eluted by 3 BV of water

4 BV 70% ethanol as the eluent. Conclusion: This process of purification technology was stable and feasible.

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