Simultaneous Determination of Geniposide and Baicalin Content in Fangfengtongsheng Pill by Double Wavelength HPLC

HOU Zhi-jian; SHI Yong-qing

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Simultaneous Determination of Geniposide and Baicalin Content in Fangfengtongsheng Pill by Double Wavelength HPLC

更新时间：2024-01-04

- Simultaneous Determination of Geniposide and Baicalin Content in Fangfengtongsheng Pill by Double Wavelength HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 24, Pages: 80-82(2011)
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- Published：2011
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HOU Zhi-jian, SHI Yong-qing. Simultaneous Determination of Geniposide and Baicalin Content in Fangfengtongsheng Pill by Double Wavelength HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 80-82.
DOI：

HOU Zhi-jian, SHI Yong-qing. Simultaneous Determination of Geniposide and Baicalin Content in Fangfengtongsheng Pill by Double Wavelength HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 80-82. DOI：

摘要

目的 :建立同时测定防风通圣丸中栀子苷和黄芩苷含量的方法。方法 :采用双波长HPLC

Hibar C18柱(4.6 mm×150 mm

5 μm)

流动相乙腈-0.2%磷酸水溶液

梯度洗脱

流速1.0 mL·min-1

检测波长分别为240 nm(栀子苷)、278 nm(黄芩苷)

柱温30 ℃。结果 :栀子苷和黄芩苷进样量分别在0.16~0.8 μg

0.24~1.2 μg线性关系良好

平均回收率分别为96.0%

100.7%

RSD分别为2.96%

2.86%。结论 :该法简便、准确、重复性好

可用于同时测定防风通圣丸中栀子苷和黄芩苷含量。

Abstract

Objective :To establish a method for simultaneously determining geniposide and baicalin in Fangfengtongsheng pill. Method :A double wavelength RP-HPLC method was developed. The analysis was performed on a Hibar C18 column (4.6 mm×150 mm

5 μm)with gradient elution using acetoniterile-0.2% phosphoric acid

the flow rate was 1.0 mL·min-1. Geniposide and baicalin were detected at 240 nm and 278 nm respectively. The column temperature was 30 ℃. Result :The calibration curves of geniposide and baicalin showed good linearity in the ranges of 0.16-0.8 μg and 0.24-1.2 μg. The average recoveries were 96.0% and 100.7% with RSD of 2.96% and 2.86%. Conclusion :The method is simple

accurate and reproducible for simultaneous determining geniposide and baicalin in Fangfengtongsheng pill.

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Related Author

LU Mei1

XIE Dong2

XIAN Yin-li3

SHI Yong-qing

SHI Yong-hua

ZHANG Pei-qin

SU Song-bai

ZHANG Jian-ling

Related Institution

The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine

Research Center for Differentiation and Development of Traditional Chinese Medicine （TCM） Basic Theory， Jiangxi Province Key Laboratory of TCM Etiopathogenisis， Jiangxi University of Chinese Medicine

Henan Academy of Chinese Medicine

The Second Clinical Medical College， Henan University of Chinese Medicine

State Key Laboratory of Functions and Applications of Medicinal Plants，School of Pharmaceutical Sciences，Engineering Center of Microbiology and Biochemical Pharmacy，Experimental Animal Center，Guizhou Medical University

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