Determination of Molecular Weight and Analysis of Monosaccharide Composition in Isolation of Polysaccharide from Imperata Cylindrica
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Determination of Molecular Weight and Analysis of Monosaccharide Composition in Isolation of Polysaccharide from Imperata Cylindrica
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 2, Pages: 80-82(2012)
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Published:2012
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ZOU Yi-ke, ZHANG Ming-yue, WANG Cai-yun, et al. Determination of Molecular Weight and Analysis of Monosaccharide Composition in Isolation of Polysaccharide from Imperata Cylindrica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 80-82.
DOI:
ZOU Yi-ke, ZHANG Ming-yue, WANG Cai-yun, et al. Determination of Molecular Weight and Analysis of Monosaccharide Composition in Isolation of Polysaccharide from Imperata Cylindrica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 80-82.DOI:
Determination of Molecular Weight and Analysis of Monosaccharide Composition in Isolation of Polysaccharide from Imperata Cylindrica
Objective: To study on isolation the polysaccharide from Imperata cylindrica and to establish a method for the determination of the molecular weight of the polysaccharides in Imperata cylindrica
and to measure its composition and mole ratio through HPLC. Method: The polysaccharide in Imperata cylindrica was Isolated and purified through the way of the staged alcohol deposition. Dextran was used as standard sample to draw the molecular weight standard curve
and the molecular weight of Imperata cylindrica polysaccharides was determined by HPLC
the polysaccharide was hydroyzed by acid to analyze monosaccharide composition and the mole ratio by HPLC. Result: The molecular weight of Imperata cylindrica polysaccharide was 8 292.2. According to HPLC IC1 was composed of Rhamnose
Xylose
Fructose
Mannose and Glucose
the mole ratio of these monosaccharides was 1∶11.45∶1.26∶1.02∶59.23. Conclusion: This method is simple accurate and sensitive.
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Jiangxi University of Chinese Medicine
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