Separation and Purification Technology of Pseudo-ginsenoside F from Total Saponins in Leaves and Stems of

TIAN Xu; REN Yuan-yuan; LI Ping-ya; WANG Cui-zhu; LIU Jin-ping

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Separation and Purification Technology of Pseudo-ginsenoside F from Total Saponins in Leaves and Stems of

更新时间：2024-01-04

- Separation and Purification Technology of Pseudo-ginsenoside F from Total Saponins in Leaves and Stems of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 6, Pages: 24-26(2012)
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- Published：2012
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TIAN Xu, REN Yuan-yuan, LI Ping-ya, et al. Separation and Purification Technology of Pseudo-ginsenoside F from Total Saponins in Leaves and Stems of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 24-26.
DOI：

TIAN Xu, REN Yuan-yuan, LI Ping-ya, et al. Separation and Purification Technology of Pseudo-ginsenoside F from Total Saponins in Leaves and Stems of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 24-26. DOI：

摘要

目的: 优选从西洋参茎叶总皂苷中分离纯化拟人参皂苷F11的最佳工艺。方法: 以拟人参皂苷F11(PF11)提取率为指标

建立HPLC-ELSD检测PF11的方法

并优选AB-8大孔吸附树脂纯化西洋参茎叶总皂苷中PF11的工艺。结果: 最佳工艺为西洋参茎叶总皂苷过10倍量AB-8大孔吸附树脂柱

流速5 mL·min-1

依次用7.5 BV 30% ~33%乙醇

8 BV 35% ~40%乙醇进行洗脱

收集35% ~40%乙醇洗脱液

回收乙醇

蒸干

得PF11粗品纯度>50.0%。结论: 大孔吸附树脂分离拟人参皂苷F11方法简单、快速、可行。

Abstract

Objective: To optimize optimum separation and purification technology of pseudo-ginsenoside F11 from total saponins in leaves and stems of Panax quinquefolium. Method: PF11 was determined by HPLC-ELSD with yield of PF11 as index

and AB-8 type macroporous adsorption resin was choosen to purify PF11from total saponins in leaves and stems of P. quinquefolium. Result: Optimum technology was:total saponins in leaves and stems of P. quinquefolium passed 10 times the amount of AB-8 macroporous adsorption resin with flow rate 5 mL·min-1

eluted with 7.5 BV 30%ethanol-33%ethanol

discard eluate

then eluted with 8 BV 35%ethanol-40%ethanol

collected eluate

recovered ethanol

evaporated

purity of pseudo-ginsenoside F11 was more than 50%. Conclusion: This method of PF11was separated by macroporous adsorption resin was simple

rapid and feasible.

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Related Institution

College of Food Science and Biology， Hebei University of Science and Technology

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine

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