Objective: To study the influence of different preparation methods on the pharmacokinetics of main effective components in Guanxin formulaⅡ. Method: Beagle dogs were administrated orally with Guanxin formulaⅡand extracted effective components formula

respecttively. The plasma concentrations of danshensu and ferulic acid were assayed by LC-MS/MS after administration. The pharmacokinetic parameters were estimated by DAS2.0 pharmacokinetics software edited by SunRuiyuan

and then compared within different groups by statistic software SPSS 10.0. Result: As for danshensu

the Cmax and AUC of Guanxin formulaⅡwere significantly higher than the extracted effective components formula

with P<0.05

while Tmax and t1/2 possed no significant difference. But for ferulic acid

the Cmax of the former was significantly lower

while AUC and t1/2 with no statistic difference. Conclusion: The blood concentration and bioavailability of danshensu in Guanxin formularⅡwere higher

but for ferulic acid

they were significantly lower

which indicates that different preparation methods had different impacts on pharmacokinetics

and its parameters should be used to determine the best preparation method.