Content Determination of Diosgenin in Tongfengning Mini-pill by HPLC
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Content Determination of Diosgenin in Tongfengning Mini-pill by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 24, Pages: 71-73(2011)
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Published:2011
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WANG Zhi-hong, LI Xue-ting, WANG Pei, et al. Content Determination of Diosgenin in Tongfengning Mini-pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 71-73.
DOI:
WANG Zhi-hong, LI Xue-ting, WANG Pei, et al. Content Determination of Diosgenin in Tongfengning Mini-pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 71-73.DOI:
Content Determination of Diosgenin in Tongfengning Mini-pill by HPLC
Objective : To establish a method for determing diosgenin in Tongfengning mini-pill. Method : HPLC deternination of Diosgenin in Tongfengning mini-pill. Column: Agilenttc SB-C18 (4.6 mm×250 mm
5 μm)
mobile phase was acetonitrile-water (10 ∶90)
flow rate 1.0 mL·min-1
column temperature: 17 ℃
detection wavelength was 203 nm. Result : Diosgenin in 1.000-5.000 μg range and peak area was linear regression
r=0.999 7
the average recovery was 101.95%
RSD 0.08%. Conclusion : The method is simple and reproducible for the determination of diosgenin in decoction of Tongfengning mini-pill by HPLC.