On-line Scavenging Activity of Xiaoaiping Injection for ABTS Radicals by HPLC ()

GAO Xiang; WANG Yan; ZHU Dan-ni; LI Er-na; ZHANG Cang; YU Bo-yang

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On-line Scavenging Activity of Xiaoaiping Injection for ABTS Radicals by HPLC ()

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- On-line Scavenging Activity of Xiaoaiping Injection for ABTS Radicals by HPLC ()
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 5, Pages: 54-57(2012)
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- Published：2012
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GAO Xiang, WANG Yan, ZHU Dan-ni, et al. On-line Scavenging Activity of Xiaoaiping Injection for ABTS Radicals by HPLC ()[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(5): 54-57.
DOI：

GAO Xiang, WANG Yan, ZHU Dan-ni, et al. On-line Scavenging Activity of Xiaoaiping Injection for ABTS Radicals by HPLC ()[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(5): 54-57. DOI：

摘要

目的: 利用HPLC在线检测消癌平注射液对ABTS·+ 的清除活性

研究其抗氧化活性物质基础。方法: 消癌平注射液经HPLC在柱后与ABTS·+工作液混合并充分反应后

经过检测器记录反应信号

通过对照品鉴定各主要成分

并计算其清除率。结果: 已鉴定出新绿原酸、咖啡酸、绿原酸

其对ABTS·+的清除率分别为9.9%

22.5%

16.5%。结论: 消癌平注射液中酚酸类物质对ABTS·+有较强的清除作用;此方法可实现中药制剂抗氧化活性成分的在线检测。

Abstract

Objective: To study the scavenging activities of Xiaoaiping injection for ABTS·+ by HPLC to trace the active constituents on-line. Method: Solution of Xiaoaiping injection was passed through HPLC to enter into the PEEK coil contained the ABTS·+ working solution

and signals were recorded

and their chemical constituents were identified by standard references

and their scavenging rates were calculated. Result: Neochlorogenic acid

caffeic acid and chlorogenic acid were identified

about the scavenging activities for ABTS·+

and their scavenging rates were 9.9%

22.5%

16.5% respectively. Conclusion: Phenolic acids in Xiaoaiping injection make a significant contribution to the scavenging activities for ABTS·+. It is shown that the method for on-line detection is an advantageous tool to rapidly determine antioxidants from traditional Chinese medicine.

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