Extraction Purification and Preparation of Isoguanosine from
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Extraction Purification and Preparation of Isoguanosine from
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 6, Pages: 21-24(2012)
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Published:2012
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LI Sheng-mei, ZENG Bao, HUANG Meng-qiu, et al. Extraction Purification and Preparation of Isoguanosine from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 21-24.
DOI:
LI Sheng-mei, ZENG Bao, HUANG Meng-qiu, et al. Extraction Purification and Preparation of Isoguanosine from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 21-24.DOI:
Extraction Purification and Preparation of Isoguanosine from
purifying and preparing isoguanosine from Croton tiglium. Method: Orthogonal design was used to optimize extraction technology parameters with the content of isoguanosine as index; Studying purification process of isoguanosine with macroporous resin and preparing high purity isoguanosine by preparative liquid chromatography. Result: Optimum extraction technology of isoguanosine was extracted 3 times with 8 times the amount of 20% ethanol
40 min each time. After preliminarily purifying by macroporous resin
isoguanosine was isolated from purified extracting solution and the purity was up to 98%. Conclusion: This method was simple and easy to operate
also could be used for preprartion high purity isoguanosine samples.