Simultaneous Separation and Purification Technology of Total Flavonoids and Total Saponins from by AB-8 Macroporous Resin
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Simultaneous Separation and Purification Technology of Total Flavonoids and Total Saponins from by AB-8 Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 3, Pages: 5-8(2012)
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Published:2012
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FENG Su-xiang, MIAO Ming-san, MIAO Jin-xin, et al. Simultaneous Separation and Purification Technology of Total Flavonoids and Total Saponins from by AB-8 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 5-8.
DOI:
FENG Su-xiang, MIAO Ming-san, MIAO Jin-xin, et al. Simultaneous Separation and Purification Technology of Total Flavonoids and Total Saponins from by AB-8 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 5-8.DOI:
Simultaneous Separation and Purification Technology of Total Flavonoids and Total Saponins from by AB-8 Macroporous Resin
Objective: To discuss purification technology conditions of total flavonoids and total saponins from Llex pubescens by macroporous resin. Method:With absorption rate and transfer rate of total flavonoids and total saponins as indexes
investigated concentration of sample liquid
pH
ratio of diameter to height of macroporous resin column
ratio of macroporous resin to crude drug
concentration of eluted ethanol and other factors. Result: Optimum purification conditions were as follows:concentration of sample liquid was 0.4 g ·mL-1
pH of sample liquid was 4.5
ratio of crude drug to macroporous resin was 1∶8
ratio of diameter to height of macroporous resin column was 1∶15
total saponins were eluted by 12 BV water
10 BV 30% ethanol and 10 BV 70% ethanol respectively. Conclusion: Macroporous resin could be used to purify total flavonoids and total saponins from L. pubescens
the content of total flavonoid and total saponins reached more than 50%.