Purification of Total Flavonoids from Leaves of  Macroporous Resin

ZHU Xin-ting; LIU Yun

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Purification of Total Flavonoids from Leaves of Macroporous Resin

更新时间：2024-01-04

- Purification of Total Flavonoids from Leaves of Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 6, Pages: 13-16(2012)
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- Published：2012
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ZHU Xin-ting, LIU Yun. Purification of Total Flavonoids from Leaves of Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 13-16.
DOI：

ZHU Xin-ting, LIU Yun. Purification of Total Flavonoids from Leaves of Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 13-16. DOI：

摘要

目的: 研究大孔树脂纯化无花果叶总黄酮的工艺条件。方法: 采用静态吸附法对5种大孔树脂进行筛选

动态吸附法优选大孔树脂纯化无花果叶总黄酮工艺。结果: 无花果叶总黄酮的纯化工艺为上样浓度2 g·L-1

上样速率2 BV·h-1

上样体积3 BV

2 BV去离子水冲洗;4 BV 80%乙醇以2 BV·h-1流速洗脱

收集洗脱液;此工艺条件下无花果叶总黄酮的转移率为77.6%

样品产物中总黄酮质量分数51.3%。结论: D140型大孔树脂对无花果叶总黄酮具有良好的富集纯化效果。

Abstract

Objective: To study on purification technology conditions of total flavonoids from leaves of Ficus carica by macroporous resin. Method: Five models of macroporous resins were selected by static adsorption

purification technology of total flavonoids from leaves of F. carica with macroporous resin was optimized by dynamic adsorption. Result: Optimum purification technology was as follows:concentration of sample 2 g·L-1

velocity of sample 2 BV·h-1

volume of sample 3 BV

eluted with 2 BV deionized water and abandoned eluate

then eluted with 4BV 80% ethanol at speed of 2 BV·h-1

eluate was collected;Yield of total flavonoids was 51.3% at this technology conditions

and recovery rate of total flavonoids was 77.6%. Conclusion: D140 macroporous resin could purify total flavonoids from leaves of F. carica effectively.

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Related Institution

Affiliated Hospital of Jiangsu University

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