Objective: To establish an HPLC method for the simultaneous determination of albiflorin

paeoniflorin

2

3

5

4'-tetrahydroxystilbene-2-O-β-D-glucoside and penta-O-galloyl-β-D-glucoside in Kundan Capsule. Method: The analysis was carried out on an Agilent TC-C18 column (4.6 nm×250 mm

5 μm). The mobile phase was composed of acetonitrile (A)-methanol (B)-0.1% phosphoric acid aqueous (C) with gradient elution; the detection wavelength was set at 230 nm; the column temperature was at 30 ℃; the flow rate was 1.0 mL·min-1. Result: The calibration curves of albiflorin

paeoniflorin

2

3

5

4'-tetrahydroxystilbene-2-O-β-D-glucoside and penta-O-galloyl-β-D-glucoside were linear in the ranges of 0.02-1.84

0.03-1.65

0.03-3.44

0.01-0.81 mg (r>0.999 9) respectively. The mean recoveries of low

middle and high concentration of the four components were among 99.5%-102.6% (RSD 1.3%-2.1%)

98.5%-103.5% (RSD 0.5%-2.4%)

98.3%-104.2% (RSD 0.5%-2.2%). Conclusion: The method is accurate and sensitive

which can be used in the quality control of Kundan capsule.