Analysis of Bupleurum Chinese Obrained from Different Harvest Period in Supercritical Fluid Extraction by GC-MS Spectrometry

LEI Xue; WANG Qi-shuai; YANG Yun; LI Xiao-kun

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Analysis of Bupleurum Chinese Obrained from Different Harvest Period in Supercritical Fluid Extraction by GC-MS Spectrometry

更新时间：2024-01-04

- Analysis of Bupleurum Chinese Obrained from Different Harvest Period in Supercritical Fluid Extraction by GC-MS Spectrometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 2, Pages: 69-71(2012)
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- Published：2012
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LEI Xue, WANG Qi-shuai, YANG Yun, et al. Analysis of Bupleurum Chinese Obrained from Different Harvest Period in Supercritical Fluid Extraction by GC-MS Spectrometry[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 69-71.
DOI：

LEI Xue, WANG Qi-shuai, YANG Yun, et al. Analysis of Bupleurum Chinese Obrained from Different Harvest Period in Supercritical Fluid Extraction by GC-MS Spectrometry[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 69-71. DOI：

摘要

目的: 以河南嵩县地区北柴胡为对象

研究北柴胡超临界提取物的化学成分及动态积累规律。方法: 用超临界流体萃取法(SFE)萃取得到北柴胡超临界提取物

并运用气相-质谱联用技术(GC-MS)分析北柴胡超临界提取物中化学成分的组成。结果: 从北柴胡超临界提取物中分离得到了59个峰

并鉴定了36个相对含量较高的成分

其占提取物总量的74.32%~90.45%。提取物中脂肪酸类化合物相对含量在45.10%~80.95%;醛类在1.61%~5.65%;酮类在0.65%~1.28%;酯类在4.80%~8.12%。结论: 北柴胡中脂肪酸类、醛类、酮类及酯类成分的相对比重均随着采收期的不同而呈现明显差异

脂肪酸类在12月份相对含量达到最高

其他3种在5月份所占比重最大。此分析为北柴胡药材的种植及采收提供了一定的科学依据。

Abstract

Objective: Choose Bupluerum chinense collected from songxian

henan province as the object

to study the chemical compositions and the dynamic change law of B. chinense extracts. Method: The extracts were extracted by supercritical fluid extraction (SFE)

and the composition of chemical constituents of SFE was determined by chromatography-mass spectrometry (GC-MS). Result: Fifty-nine peaks have been separated and 36 peaks of them have been identified

which accounting for 74.32%-90.45% of total extracts. The relative content of the fatty acids which extracted by SFE-CO2 was 45.10%-80.95%

the aldehydes was 1.61%-5.65%

the ketones was 0.65%-1.28%. Conclusion: The fatty acids

the ketones and the esters in B. chinense are obviously different in different harvest period. The fatty acids reached the highest relative content on December

the others reached the highest content on May. This analysis can provide a certain scientific basis for the growth and collection of B. chinense.

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