Objective: To establish a HPLC method for the determination of schizandrin

schisantherin A

deoyschizandrin

γ-schishandrin and tanshinone ⅡA in Yixinshu tablet. Method: The Phenomenex Kinetex C18 column was used with a mobile phase of acetonitrile-water by gradient elution

the flow rate was 1 mL·min-1

the column temperature was 30 ℃

the detection wavelength was at 250 nm. Result: The linear ranges of schizandrin

schisantherin A

deoxyschizandrin

γ-schishandrin and tanshinone ⅡA were 21.56-215.6 mg·L-1(r=0.999 9)

6.500-65.00 mg·L-1 (r=1)

3.460-34.60 mg·L-1 (r=0.999 9)

6.920-69.20 mg·L-1 (r=0.999 9) and 2.400-24.00 mg·L-1 (r=0.999 9) respectively.The average recoveries of five components were 97.92% with RSD 2.34%

100.28% with RSD 2.57%

98.34% with RSD 2.73%

98.06%with RSD 1.61% and 99.13% with RSD 1.42%. Conclusion: The method is simple

sensitive and repeatable.It can be a method for quality control of Yixinshu tablet.