Objective: Establish a method of determination for flavor nucleotides. Method: Diamonsil C18 column(4.6 mm×250 mm

5 μm) was employed.Acetonitrile-water (containing 0.005 mol·L-1 tetrabutyl ammonium bromide and 0.05 mol·L-1 monobasic potassium phosphate) (2: 98) was used as mobile phase

flow rate was 1.0 mL·min-1. Ultraviolet detection wave length was 250 nm. Result: The IMP (disodium inosinate)was linear in the concentration range of 0.50-4.00 μg(r=0.999 9).The average recovery rate (n=6) was 99.12% (RSD 0.56%). The GMP(disodium 5'-guanglate) was linear in the concentration range of 0.50-4.00 μg(r=0.999 99). The average recovery rate (n=6) was 99.18% (RSD 0.45%). Conclusion: The method is accurate and reliable

which can be used to determinate the quality of IMP and GMP in flavor nucleotides.