Enrichment and Purification of Alkaloids from with D101 Macroporous Resin
|更新时间:2024-01-04
|
Enrichment and Purification of Alkaloids from with D101 Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 6, Pages: 16-20(2012)
作者机构:
作者简介:
基金信息:
DOI:
CLC:
Published:2012
稿件说明:
移动端阅览
PENG Tuo-hua, ZHANG Shao-jun, ZHONG Shi-shun, et al. Enrichment and Purification of Alkaloids from with D101 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 16-20.
DOI:
PENG Tuo-hua, ZHANG Shao-jun, ZHONG Shi-shun, et al. Enrichment and Purification of Alkaloids from with D101 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 16-20.DOI:
Enrichment and Purification of Alkaloids from with D101 Macroporous Resin
Objective: To resarch chemical composition change after enrichment and purification of alkaloids from Aconitum carmichaelii with D101 macroporous resin. Method: Taking transfer rate of total alkaloids
diester diterpenoid alkaloids
aconitine
hypaconitine and mesaconitine as indexes
before and after purification
the content of alkaloids from extracts of A. carmichaelii was determined by UV and HPLC
ingredients of alkaloids differentiated by TLC. Result: Alkaloids from A. carmichaelii was enriched by D101 macroporous resin
transfer rate and purity of total alkaloids were 83.70%
67.34%
respectively;Transfer rate of aconitine
hypaconitine and mesaconitine were 77.78%
94.12%
52.63%;It showed 6 similar biological spots by TLC comparison
this indicated that there was no significant difference before and after enrichment of alkaloids composition. Conclusion: D101 type macroporous resin could effectively enhance purity of total alkaloids from A. carmichaelii with high transfer rate of alkaloids