Determination of Orientin and Isoorientin in Different Harvest Times of by HPLC
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Determination of Orientin and Isoorientin in Different Harvest Times of by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 2, Pages: 62-64(2012)
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Published:2012
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DOU Yan, ZHAI Yan-jun, TONG Miao-miao, et al. Determination of Orientin and Isoorientin in Different Harvest Times of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 62-64.
DOI:
DOU Yan, ZHAI Yan-jun, TONG Miao-miao, et al. Determination of Orientin and Isoorientin in Different Harvest Times of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(2): 62-64.DOI:
Determination of Orientin and Isoorientin in Different Harvest Times of by HPLC
Objective: To establish an HPLC method for the simultaneous determination of orientin and isocrientin in different harvest times of Polygonum orientale. Method: Orientin and isoorientin were separated on Agilent C18(4.6 mm×150 mm
5μm) column and detected at 350 nm.The mobile phase was acetonitrile-0.3% phosphoric with gradient elution.The flow rate was 1.0 mL·min-1. Result: Orientin and isoorientin were linear within the range of 0.068 2-0.682 μg(r=0.999 7)
0.386-3.860 μg(r=0.999 3)respectively.The average recovery was 99.94%(1.08%)
99.67%(1.09%). Conclusion: The method is simple
rapid
and it can be used for the quality control of P. orientale.