The Methodology Study on Mollugin Content Determination in Radix Rubiae Extracts
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The Methodology Study on Mollugin Content Determination in Radix Rubiae Extracts
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 6, Pages: 94-96(2012)
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Published:2012
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PU Yi-qiong, WANG Bing, ZHANG Tong, et al. The Methodology Study on Mollugin Content Determination in Radix Rubiae Extracts[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 94-96.
DOI:
PU Yi-qiong, WANG Bing, ZHANG Tong, et al. The Methodology Study on Mollugin Content Determination in Radix Rubiae Extracts[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 94-96.DOI:
The Methodology Study on Mollugin Content Determination in Radix Rubiae Extracts
Objective: To establish a quantitative HPLC method for the determination of mollugin in extract from Rubia cordifolia L. Method: The determination was carried out on a Ultimate XB-C18 column at 25 ℃ with methanol-acetonitrile-0.2%phosphoric acid (25:50:25) as mobile phase and detection wavelength at 250 nm. The flow rate was 1.0 mL·min-1. Result: The good linearity was obtained with the correlation coefficients ranged of 3.566-456.4 mg·L-1(r=0.999 9). The average recovery rate was 98.97% (RSD 2.29%). Conclusion: This method is accurate and reliable with easy operation
which can be used in the quality control of extracts from R. cordifolia.