Determination of Adenosine in  from Different Cultivation Areas by HPLC

LI Jun; YUE Yi-heng; ZHANG Li-ping; ZHENG Yuan-miao; GUO Hai-quan; ZHANG Xiao-feng; BAI Yan

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Determination of Adenosine in from Different Cultivation Areas by HPLC

更新时间：2024-01-04

- Determination of Adenosine in from Different Cultivation Areas by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 24, Pages: 55-57(2011)
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- Published：2011
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LI Jun, YUE Yi-heng, ZHANG Li-ping, et al. Determination of Adenosine in from Different Cultivation Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 55-57.
DOI：

LI Jun, YUE Yi-heng, ZHANG Li-ping, et al. Determination of Adenosine in from Different Cultivation Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 55-57. DOI：

摘要

目的 :分析不同产地的山药中腺苷的含量

为山药质量标准的建立提供参考。方法: 采用高效液相色谱法

SunFire C18色谱柱(4.6 mm×250 mm

5 μm);磷酸盐缓冲液(pH 6.5)-甲醇(85 ∶15)为流动相

流速 1.0 mL·m in-1;检测波长 260 nm。结果: 10批不同产地山药中腺苷含量介于0.035 9~0.104 0 mg·g-1。结论: 该方法操作简便

结果稳定可靠

可用于山药的质量评价。

Abstract

Objective: To determine content of adenosine in Dioscorea opposita from the various cultivation areas. Method : A SunFire C18 Colum (4.6 mm×250 mm

5 μm) was used with Methanol- phosphate buffer(15 ∶85)as the mobile phase. The detective wavelength was set at 260 nm and the flow rate was 1.0 mL·min-1. Result: Ten batches of the samples were analyzed. The content of adenosine ranged from 0.035 9-0.104 0 mg·g-1. Conclusion : The method was simple

reproducible and reliable. It can be used to control the quality of D. opposita.

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