Determination of Gallic Acid and Ellagic Acid in  by Variable Wavelength of HPLC

LIU Yan; XIONG Wei; TIAN Ji; HE Bing

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Determination of Gallic Acid and Ellagic Acid in by Variable Wavelength of HPLC

更新时间：2024-01-04

- Determination of Gallic Acid and Ellagic Acid in by Variable Wavelength of HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 6, Pages: 84-86(2012)
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- Published：2012
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LIU Yan, XIONG Wei, TIAN Ji, et al. Determination of Gallic Acid and Ellagic Acid in by Variable Wavelength of HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 84-86.
DOI：

LIU Yan, XIONG Wei, TIAN Ji, et al. Determination of Gallic Acid and Ellagic Acid in by Variable Wavelength of HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 84-86. DOI：

摘要

目的: 建立同时测定泸州龙眼肉、核、皮中没食子酸和鞣花酸含量的HPLC方法。方法: 依利特 Sino Chrom ODS-BP (4.6 mm×250 mm

5 μm)

流动相乙腈-0.1%磷酸溶液

梯度洗脱

改变波长测定(0~11.5 min

215 nm

测定没食子酸;11.5~25 min

255 nm

测定鞣花酸)

柱温30 ℃

流速1 mL·min-1

进样量10 μL。结果: 没食子酸在0.229~2.29 μg

鞣花酸在0.412 5~4.125 μg峰面积与进样量有良好的线性关系(r＝0.999 9)

平均回收率(n＝9)分别为98.27%(RSD 0.63%)

98.31%(RSD 0.88%)。结论: 该方法灵敏、准确、重复性好

可用于泸州龙眼中没食子酸和鞣花酸的含量测定。

Abstract

Objective: To establish a method for determination of the gallic gallic acid and ellagic ellagic acid indifferent part of Dimocarpus longan. Method: An HPLC method was performed on a SinoChrom ODS-BP (4.6 mm×250 mm

5 μm) at 30 ℃. The mobile phase was acetonitrile-0.1% phosphoric acid with gradient elution and changeable wavelength detection(0-11.5 min

215 nm for gallic acid and 11.5-25 min

255 nm for ellagic acid).The flow rate was 0.8 mL·min-1 and the sample size was 10 μL. Result: The calibration curve of gallic gallic acid and ellagic ellagic acid showed good linearity over the range of 0.229-2.29 μg(r=0.999 9)

0.412 5-4.125 μg(r＝0.999 9).The average recoveries were 98.27%(RSD 0.63%)

98.31%(RSD 0.88%). Conclusion: This method is sensitive

accurate

reproducible

it can be used for the quality control of longan.

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Related Author

LI Yan-fang1

XIA Quan2

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Related Institution

Gansu Provincial Institute of Food and Drug Control,Gansu Province Traditional Chinese Medicine Quality and Safety Evaluation of Engineering Technology Research Center

Guiyang Collage of Traditional Chinese Medicine

Guiyang College of Traditional Chinese Medicine

School of Life Sciences，Beijing University of Chinese Medicine

Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine

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