Thin-layer Chromatography of Unsaponifiable Matter from 36 Oily Chinese Materia Medica

CHAO Zhi-mao; ZHANG Nan; TANG Chun-feng; WANG Chun

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Thin-layer Chromatography of Unsaponifiable Matter from 36 Oily Chinese Materia Medica

更新时间：2024-01-04

- Thin-layer Chromatography of Unsaponifiable Matter from 36 Oily Chinese Materia Medica
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 3, Pages: 79-83(2012)
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- Published：2012
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CHAO Zhi-mao, ZHANG Nan, TANG Chun-feng, et al. Thin-layer Chromatography of Unsaponifiable Matter from 36 Oily Chinese Materia Medica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 79-83.
DOI：

CHAO Zhi-mao, ZHANG Nan, TANG Chun-feng, et al. Thin-layer Chromatography of Unsaponifiable Matter from 36 Oily Chinese Materia Medica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(3): 79-83. DOI：

摘要

目的: 对油性中药的不皂化物进行薄层色谱的研究

建立统一的鉴别方法。方法: 36种油性中药

以5%氢氧化钾甲醇溶液进行皂化反应

以1 mL含有1 g药材浓度的不皂化物样品溶液薄层点样

以α-菠菜甾醇、β-谷甾醇、齐墩果酸和栝楼仁二醇为对照品进行对照

以薄层色谱的显色斑点进行鉴别。结果: 各油性中药的不皂化物质在同一条件下均能显示明显的薄层色谱斑点。急性子、牛蒡子、茺蔚子、肉豆蔻、蛇床子、瓜蒌子、女贞子均显示α-菠菜甾醇的斑点

除急性子、木鳖子、栝楼种子外的所有供试品均显示β-谷甾醇的斑点

肉豆蔻、瓜蒌子、女贞子均显示齐墩果酸的斑点

栝楼种子、双边栝楼种子均显示栝楼仁二醇的斑点。结论: 牛蒡子、茺蔚子、肉豆蔻、蛇床子、女贞子含有α-菠菜甾醇

肉豆蔻、瓜蒌子含有齐墩果酸

郁李仁含有β-谷甾醇等

均系首次报道。油性中药的不皂化物质可以在同一条件下进行薄层色谱的比对。

Abstract

Objective: To establish an identification of the oily Chinese materia medica by thin-layer chromatography (TLC). Method: Thirtysix samples of oily Chinese materia medica were collected

saponified with 5% KOH in methanol

and made test solutions at a concentration of 1 g crude drug in 1 mL ethyl acetate. α-Spinasterol

β-sitosterol

oleanolic acid

and karounidiol were taken as reference solutions. The spots on the TLC chromatogram were identified. Result: All of the oily Chinese materia medica has a good TLC chromatogram. Impatientis Semen

Arctii Fructus

Leonuri Fructus

Myristaticae Semen

Cnidii Fructus

Ligustri lucidi Fructus

and Trichosanthis Semen show a spot of α-spinasterol. All of the Chinese materia medica shows a spot of β-sitosterol but Impatientis Semen

Momordicae Semen

and Seed of Trichosanthes kirilowii. Myristaticae Semen

Ligustri lucidi Fructus

and Trichosanthis Semen show a spot of oleanolic acid. Trichosanthis Semen

seeds of both Trichosanthes kirilowii and Trichosanthes rosthornii

show a spot of karounidiol. Conclusion: It’s the first study to demonstrate that Arctii Fructus

Leonuri Fructus

Myristaticae Semen

Cnidii Fructus

and Ligustri lucidi Fructus contain α-spinasterol

Myristaticae Semen and Trichosanthis Semen contains oleanolic acid

and Semen Pruni contain β-sitosterol. The oily Chinese materia medica can be identified by the TLC under same condition.

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Related Institution

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Dept.of Pharmacy,The First Affiliated Hospital of Xinjiang Medical University

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