Objective: To establish a HPLC method for simultaneous determination of preservatives in traditional chinese oral liquid preparation. Method: The chromatographic separation was achieved on a Kromasil C18(4.6 mm×150 mm

5 μm) column with acetonitrile-0.02 mol ·L-1 ammonium acetate solution as mobile phase(gradient elution).The flow rate was 1.0 mL ·min-1

the detective wavelength was set at 240 nm

and the column temperature was maintained at 30 ℃. Result: Benzoic acid

sorbic acid and parahydroxybenzoate ester have a good resolution and linear correlation in certain range

respectively (r>0.999). The average recoverieswere 95.5%-100.8% (RSD 1.5%-1.8%). Conclusions: The method is simple

reliable and accurate for determination of preservatives in traditional chinese oral liquid preparation.