Objective: To study the HPLC fingerprint of Lonicerae Japonicae Flos and establish a chromatographic standard for the quality control as well as to comparative study the HPLC fingerprint among various farm cultivars. Method: The HPLC method was used. The gradient elution was adopted acetonitrile and 1% acetic acid.The 'similarity evaluation system for chromatographic fingerprint of TCM’ algorithm was adopted in combination with the clustering analysis to study ten flos lonicerae’s thirteen characteristic common peaks

and to evaluate the quality and dividide the farm cultivars of flos lonicerae. Result: The HPLC fingerprint of Lonicerae Japonicae Flos were established and the differences of HPLC fingerprint among the farm cultivars of Lonicerae Japonicae Flos produced in Shandong were compared. Conclusion: The HPLC fingerprint method summaried in this article can be used as the typical chromatography and provide conference evidence for dividing the Cultivars resource of Lonicerae Japonicae Flos.