Objective: To study effect of ethanol-extractions of Salvia miltiorrhiza

Syzygium aromaticum

Magnolia officinalis

Louicera japonica

Rheum palmatum this 5 kinds of traditional Chinese medicine on bacteriostasis activity to Propionibacterium acnes and Staphylococcus aureus

and then investigate different proportion of the ethanol-extractions by orthogonal test on antimicrobial effect of both Gram positive bacteria. Method: The ethanol-extractions of S. miltiorrhiza

S. aromaticum

M. officinalis

L. japonica

R. palmatum were enriched to 0.2 g ·mL-1. Inhibition zone diameters were measured to evaluate the bacteriostatic effects and the different ethanol-extraction compatibility. Result: Propionibacterium acne was highly sensitive to S. miltiorrhiza ethanol-extraction with the inhibition zone diameter of (23.27±3.68) mm. It showed moderate sensitivity to Syzygium aromaticum and Magnolia officinalis ethanol-extractions

the inhibition zone diameters were (18.82±2.27) and (17.78±1.88) mm accordingly. Staphylococcus aureus was highly sensitive to S. aromaticum and M. officinalis ethanol-extractions

the inhibition zone diameters were (21.94±0.74) and (19.52±1.09) mm respectively. It showed moderate sensitivity to R. palmatum and S. miltiorrhiza ethanol-extractions

the inhibition zone diameters were (14.43±2.24) and (16.01±0.88) mm. The best mixed proportion of alcohol extract of S. miltiorrhiza

M. officinalis and S. aromaticum was 1 ∶1 ∶1. The inhibition zone diameters of the compound to Propionibacterium acne and Staphylococcus aureus were (20.07±1.26) and (20.57±0.38) mm respectively. Conclusion: Alcohol extracts of S. miltiorrhiza

M. officinalis

S. aromaticum have high antibacterial activity to two species of bacteria. The best mixed propotion for alcohol extracts of S. miltiorrhiza

M. officinalis

S. aromaticum on antibacterial activity to two bacteria appears tobe 1 ∶1 ∶1.