Objective: To observe the regulatory effect of Gouteng San on the proliferation and apoptosis of NIH-3T3 cell lines. Method: NIH-3T3 cells were cultivated in DMEM with 10% fetal calf serum and three groups such as youth

blank and model group were used. Cells at generation 30 were treated with hydrogen peroxide (H2O2) and then β-galactosidase dyeing kit was applied to observe cell morphology and aging indications to assure whether the aging mode was successfully established.Oral administration of Gouteng San was started for three constitutive days

twice daily. One hour after last administration

rats were anesthetized

and we drew blood from abdominal aorta for the preparation of Gouteng San medicated serum. NIH-3T3 cells were treated with Gouteng San medicated serum to observe cell morphology

aging indications

proliferation and apoptosis. Result: The cell morphology of model was changed and the proportion of cell senescence was increased significantly in model group than in normal control group(P<0.05).Therefore the aging model was successfully established by inducing NIH-3T3 cells aging via oxidative stress with hydrogen peroxide (H2O2). Gouteng San medicated serum can significantly attenuate the change of cell morphology

improve aging indications

foster proliferation

(P<0.01) and reduce cell apoptosis(P<0.01)

with a distinct effect at 10 times doses. Conclusion: Gouteng San can promote proliferation and reduce apoptosis

thus prevent aging.