Objective: To observe the effect of Yigan powder on acute hepatic injury induced by D-galactosamine (D-GalN) in mice. Method: Seventy-two mice were randomly divided into 6 groups (n=12): normal control group

model control group

positive control group and three dose groups of Yigan powder (38.4

19.2

9.6 g·kg-1).The mice in treatment groups were administered medicine respectively via gavages

while the others in control and model groups were treated with the same volume of normal saline.After seven days all the mice except those in the normal control group were treated with intraperitoneal injection of 10% D-GalN (850 mg·kg-1).Twenty-four hours later

the mice were killed and the blood sample and liver、spleen tissue were taken.The index of liver and spleen was calculated.Liver pathological changes were observed and the activities of serum ALT

AST were determined. Result: Acute liver injury could be significantly reduced by Yigan powder.Serum ALT

AST activity

liver and spleen index were significantly lowered compared with those in the model group (P<0.05).Yigan powder could improve the acute liver injury in a dose dependent manner. Conclusion: Yigan powder had protective effect on acute hepatic injury induced by D-galactosamine.