Objective: To establish an analysis method to determine astragaloside in Tibetan medicine Preparation Ganxianxiao granules by SPE-HPLC-ELSD. Method: Samples were processed by the SPE

and determined on an Alltima C18 column(4.6 mm×250 mm

5 μm)

the mobile phase consisted acetonitrile-water (33∶67) with flower rate at 1.0 mL ·min-1 and column temperature at 35 ℃

while drift tube temperature was 110 ℃ of ELSD

by the carrier flow was 2.7 L ·min-1

and injection volume was 20 μL. Result: The linear ranges of astragaloside was 1.014-20.280 μg (r=0.999 3)

with the average recovery were of 100.1% (RSD 2.6%). Conclusion: The method is simple

accurate

reproducible and it can be used for the quality control of Tibetan medicine Preparation Ganxianxiao granules.