Distribution of Effective Components in Different Parts of

ZHAO Xi-xian; ZHAO Ming-shu; CHEN Yu-ting; GONG Wei-hong; LI Rong

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Distribution of Effective Components in Different Parts of

更新时间：2024-01-04

- Distribution of Effective Components in Different Parts of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 20, Pages: 111-113(2011)
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- Published：2011
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ZHAO Xi-xian, ZHAO Ming-shu, CHEN Yu-ting, et al. Distribution of Effective Components in Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 111-113.
DOI：

ZHAO Xi-xian, ZHAO Ming-shu, CHEN Yu-ting, et al. Distribution of Effective Components in Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 111-113. DOI：

摘要

目的: 分析丹参有效成分在不同部位的分布。方法: 采用HPLC

Shim-pack VP-ODS C18色谱柱(4.6 mm×150 mm

5 μm)

乙腈-0.02%磷酸水为流动相进行梯度洗脱

检测波长270

280 nm

流速1.0 mL·min-1。分别测定丹参粗皮部分

去粗皮部分和原药材有效成分的含量。结果: 丹参有效成分的分布部位不同

隐丹参酮、丹参酮ⅡA、原儿茶醛、咖啡酸主要存在于粗皮部分

丹参素和丹酚酸B主要分布在去粗皮部分;栽培丹参粗皮中隐丹参酮、丹参酮ⅡA、原儿茶醛、咖啡酸、丹参素和丹酚酸B的含量分别是去粗皮部分含量的1 933.6%

1 554.7%

700%

268.4%

56.6%

61.1%;野生丹参粗皮中隐丹参酮、丹参酮ⅡA、原儿茶醛、咖啡酸、丹参素和丹酚酸B的含量分别是去粗皮部分含量的1 393.0%

1 822.0%

235.7%

150%

62.8%

90.4%;栽培丹参中丹酚酸B、隐丹参酮、丹参酮ⅡA含量较高。结论: 丹参不同部位成分的差异证实丹参传统等级划分的科学性

可以指导我们合理地利用丹参资源。

Abstract

Objective: To analyze the active ingredient distribution in Salvia different parts. Method: An HPLC was applied using dual wavelength detection and analytical column of Shim-pack VP-ODS C18 (4.6 mm×150 mm

5 μm). The mobile phase was consisted of acetonitrile-0.02% phosphoric acid solution by gradient elution. The flow rate was 1.0 mL·min-1 with the detection wavelength at 270 nm and 280 nm.The active ingredients were measured by HPLC in Salvia rough bark part

the part without the rough bark and the original medicinal part. Result: The distribution of Salvia active ingredients is different

of which cryptotanshinone

tanshinone ⅡA

protocatechuic aldehyde

caffeic acid mainly were found in the rough bark part and danshensu and Sal B were mainly distributed in the part without the rough bark. In cultivation Salvia

the content of cryptotanshinone

tanshinone ⅡA

protocatechuic aldehyde

caffeic acid

danshensu and Sal B in the rough bark part were 1 933.6%

1 554.7%

700%

268.4%

56.6% and 61.1% compared with that in the part without the rough bark respectively. In wild Salvia

the content of cryptotanshinone

tanshinone ⅡA

protocatechuic aldehyde

caffeic acid

danshensu and Sal B in the rough bark part were 1 393.0%

1 822.0%

235.7%

150%

62.8% and 90.4% compared with that in the part without the rough bark respectively. The content of Sal B

cryptotanshinone

tanshinone ⅡA in cultivation Salvia was high. Conclusion: The differences in composition in different parts of Salvia confirmed the traditional classification scientific

and can guide our rational use of Salvia resources.

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Related Institution

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