Objective: To study the killer activity of cytokine-induced killer (CIK) cells treated with Chinese medicine polysaccharides against the ovarian cancer cell line SKOV3 under different conditions

and explore the possibility of adoptive immunotherapy combined with Chinese medicine in the treatment of ovarian cancer. Method: The experiment were divided into four groups

such as CIK group

PBDC-CIK group

SKOV3Ag-PBDC-CIKgroup and SKOV3Ag-ADC-CIKgroup. CIK cells derived from the peripheral blood (PB) were induced by cytokine and grifola polysaccharide (100 mg·L-1)

pachyman (100 mg·L-1) and astragalan (100 mg·L-1).while dendritic cells (DC) derived from PB and ascites by SKOV3 tumor cell lysate. The cytotoxic activities of CIK group

PBDC-CIK group

SKOV3Ag-PBDC-CIK group and SKOV3 Ag-ADC-CIK group against SKOV3 were analyzed

as well as the proliferation of CIK cells between groups. Result: ①The proliferation rate of CIK cells co-cultured with DC significantly increased.(P<0.01) ②CIK cells induced by Chinese medicine polysaccharides were cytotoxic to SKOV3 cells

which increased with effector: target (E ∶T) ratios

which were(9.12±0.21)%、(10.15±0.27)%、(11.20±0.34)% and (12.73±0.43)% (P<0.05)

③ The cytotoxic activities of SKOV3Ag-PBDC-CIK group against SKOV3 cells were significantly higher than that of both DC-CIK group and CIK groups (P<0.05

P<0.01). ④ There was no significant difference in killing activity between SKOV3Ag-PBDC-CIK group and SKOV3Ag-ADC-CIK group. Conclusion: DC pulsed with tumor lysate antigens of SKOV3 could enhance the proliferation and cytotoxicity of CIK cells induced by Chinese medicine polysaccharides. And DC derived from ascites could serve as an important source in adoptive immunotherapy.