Inhibitive Effect of Magnolol Against MPTP-Induced Apoptosis in PC12 Cells

YAN Yin-gen; LIN Quan-feng; XU Yuan; HUANG Xiao-li

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Inhibitive Effect of Magnolol Against MPTP-Induced Apoptosis in PC12 Cells

更新时间：2024-01-04

- Inhibitive Effect of Magnolol Against MPTP-Induced Apoptosis in PC12 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 18, Pages: 223-225(2011)
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- Published：2011
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YAN Yin-gen, LIN Quan-feng, XU Yuan, et al. Inhibitive Effect of Magnolol Against MPTP-Induced Apoptosis in PC12 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 223-225.
DOI：

YAN Yin-gen, LIN Quan-feng, XU Yuan, et al. Inhibitive Effect of Magnolol Against MPTP-Induced Apoptosis in PC12 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(18): 223-225. DOI：

摘要

目的: 探讨厚朴酚(mangolol

Mag)对1-甲基-4-苯基-1

6-四氢吡啶(MPTP)诱导PC12细胞凋亡的抑制作用及可能作用机制。方法: 将厚朴酚(终浓度0.1

10 μmol ·L-1)或/和MPTP(终浓度50

100

150

200 μmol ·L-1)加入到培养的PC12细胞中。四甲基偶氮唑盐法(MTT法)检测细胞增殖活性

用流式细胞仪检测细胞凋亡率

以及Western-blot法检测Bax和Bcl-2的蛋白表达变化。结果: 在加入不同浓度的MPTP处理细胞24 h

细胞增殖活性渐次降低

而厚朴酚0.1~10 μmol ·L-1预处理1 h可明显减轻MPTP导致的PC12细胞的损伤

抑制细胞凋亡

以及Bcl-2/Bax比值的改变。结论: 厚朴酚对MPTP诱导的PC12细胞凋亡损伤有一定的抑制作用

其作用机制涉及到影响细胞内Bcl-2/Bax蛋白的表达。

Abstract

Objective: To investigate the inhibition of magnolol on the apoptosis of PC12 cell induced by MPTP and its mechanism. Method: Magnolol (final concentration 0.1

10 μmol ·L-1) and /or MPTP(final concentration 50

100

150

200 μmol ·L-1) were added into the culture of PC12 cells. The cell viability was detected with MTT method

apoptosis was measured by flow cytometry and Bcl-2/Bax ratio was detected by western blot. Result: Cell viability was declined sharply by MPTP with different concentrations

the pre-treatment of magnolol 0.1-10 μmol ·L-1 could significantly increase the PC12 cell viability

apoptosis and the decrease in the Bcl-2/Bax ratio. Conclusion: Magnolol may inhibit the cell damage induced by MPTP and the mechanism is likely related to inhibition to expression of Bcl-2/Bax.

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