Effect of Different Extracts From  on Cultureing Osteoblasts

YU Hai-tao; JU Cheng-guo; ZHANG Qi; JIA Tian-zhu

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Effect of Different Extracts From on Cultureing Osteoblasts

更新时间：2024-01-04

- Effect of Different Extracts From on Cultureing Osteoblasts
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 24, Pages: 36-39(2011)
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- Published：2011
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YU Hai-tao, JU Cheng-guo, ZHANG Qi, et al. Effect of Different Extracts From on Cultureing Osteoblasts[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 36-39.
DOI：

YU Hai-tao, JU Cheng-guo, ZHANG Qi, et al. Effect of Different Extracts From on Cultureing Osteoblasts[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 36-39. DOI：

摘要

目的 :探索狗脊不同提取部位对体外培养成骨细胞的增殖作用;比较狗脊生、制品对细胞增长的影响。方法 :用狗脊生制品的石油醚层、氯仿层、乙酸乙酯层、正丁醇层及粗多糖与成骨细胞共同培养;并且将各提取部位给大鼠灌胃

取大鼠含药血清与成骨细胞共同培养

以MTT法检测细胞的增殖。结果 :各提取部位与细胞共同培养试验和含药血清与细胞共同培养试验中

均以狗脊正丁醇提取部位对细胞增殖作用最明显

且制品强于生品。结论 :狗脊炮制品正丁醇提取物能够显著促进成骨细胞增殖。

Abstract

Objective :To explore the effects of different extracts from Cibotium barometz on proliferation of Osteoblasts.To compare the effects on proliferation of osteoblasts between crude and processed products

to explore the mechanism of anti-osteoporosis for C. barometz. Method :Osteoblasts with petroleum ether layer extract

chloroform layer extract

ethylacetate layer extract

butanol layer extract and polysaccharide from crude and processed products of C. barometz were cultured respectively. Meanwhile

after the rats were fed with each layer extract

the rat serum together with osteolastes was cultured. The proliferation was tested by MTT method. Result :In the different extract with osteoblasts test and the rat serum with osteoblasts test

the butanol layer extract had the most significant effect on cell proliferation of osteoblasts. And the effect of the processed product was more significant than that of crude product. Conclusion :The butanol layer extract from processed products of C. barometz had the most significant effect on cell proliferation of Osteoblasts.

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Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine

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