Effects of Danqing Soft Capsule on Insulin Resistance and Leptin Resistance in Rats with Non-alcoholic Fatty Liver Disease
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Effects of Danqing Soft Capsule on Insulin Resistance and Leptin Resistance in Rats with Non-alcoholic Fatty Liver Disease
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 1, Pages: 188-191(2012)
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Published:2012
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LIU Rui, LI Jin-ping, WU Juan-juan, et al. Effects of Danqing Soft Capsule on Insulin Resistance and Leptin Resistance in Rats with Non-alcoholic Fatty Liver Disease[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 188-191.
DOI:
LIU Rui, LI Jin-ping, WU Juan-juan, et al. Effects of Danqing Soft Capsule on Insulin Resistance and Leptin Resistance in Rats with Non-alcoholic Fatty Liver Disease[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 188-191.DOI:
Effects of Danqing Soft Capsule on Insulin Resistance and Leptin Resistance in Rats with Non-alcoholic Fatty Liver Disease
Objective :To observe the effects of Danqing soft capsule (DSC) on insulin resistance and leptin resistance in rats with non-alcoholic fatty liver disease (NAFLD). Method :SD rats were randomly divided into control group
model group
DSC high dose group
medium dose group
low dose group
and polyene phosphatidyl choline (PPC) group. rat non-alcoholic fatty liver model was established with high fat diet.DSC high dose group
medium dose group and low dose group were administrated with different concentrations
which are respectively equivalent to petroleum ether extract of salvia 0.456
0.228
0.114 g ·kg-1. PPC group was treated with PPC by 0.159 g ·kg-1ig.Control group and model group were given the same volume (1.2 mL ·kg-1) of distilled water ig. After treatment for 4 weeks
blood glucose was measured; levels of serum insulin and leptin were assayed by ELISA assay; expression of leptin receptor in the liver was observed by immunohistochemical staining; the index of insulin resistance was calculated. Result : Concentrations of FGB
FINS and HOMA-IR in control group
TCM groups and western medicine group were significantly lower than those of model group (P<0.01
P<0.05); the levels of leptin in serum and expression of ob-R protein in liver of control group
TCM groups and western medicine group were significantly lower than those of model group (P<0.01
P<0.05). Conclusion :DSC can improve IR and leptin resistance in rats with NAFLD.