Determination of Oleanolic Acid and Ursolic Acid in Radix Actinidiae by RP-HPLC

DI Xue; WANG Hai-bo; ZHAI Yan-jun; PAN Lei

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Determination of Oleanolic Acid and Ursolic Acid in Radix Actinidiae by RP-HPLC

更新时间：2024-01-04

- Determination of Oleanolic Acid and Ursolic Acid in Radix Actinidiae by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 1, Pages: 66-68(2012)
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- Published：2012
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DI Xue, WANG Hai-bo, ZHAI Yan-jun, et al. Determination of Oleanolic Acid and Ursolic Acid in Radix Actinidiae by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 66-68.
DOI：

DI Xue, WANG Hai-bo, ZHAI Yan-jun, et al. Determination of Oleanolic Acid and Ursolic Acid in Radix Actinidiae by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 66-68. DOI：

摘要

目的: 建立藤梨根药材中熊果酸、齐墩果酸的含量测定方法

对不同品种、产地药材进行测定。方法 :采用反向高效液相色谱法

色谱柱为ZORBA×300SB-C18 (4.6 mm×250 mm

5 μm)

流动相甲醇-0.5%醋酸胺(76 ∶24)

流速为1.0 mL ·min-1

柱温25 ℃

检测波长210 nm。结果 :熊果酸在0.492~2.460 μg线性关系良好(r=0.999 7)

平均回收率为97.6%

RSD 1.8%;齐墩果酸在0.448~2.240 μg线性关系良好(r=0.999 9)

平均回收率为98.7%

RSD 0.6%。结论: 该方法简单、准确并且专属性强

可作为藤梨根药材质量控制方法。

Abstract

Objective: HPLC method was established for determination of ursolic acid and oleanolic acid in Radix Actinidiae. Method: HPLC was carried out on ZORBA×300SB-C18 (4.6 mm×250 mm

5 μm) at 25 ℃ using methanol-0.5% ammonium acetate (76 ∶24) as mobile phase with a flow rate of 1.0 mL ·min-1 and detection wavelength was at 210 nm. Result: Linear correlation (r = 0. 999 7) of the peak area and the concentration of ursolic acid over the concentration range 0.492-2.460 mg ·L-1 was obtained. Linear correlation (r = 0. 999 9) of the peak area and the concentration of oleanolic acid over the concentration range 0.448-2.240 mg ·L-1 was obtained. The average recovery was 97.6%

98.7% and RSD was 1.8%

0.6%. Conclusion: The method is simple

rapid and accurate. It can be used for the quality control of Radix Actinidiae.

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