Effects of Extract of Grifola Frondosa on Bcl-2 and Bax in Spleen of Spleen Deficiency Mice

LEI Ping; MA Xian-de; GUAN Hong-quan

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Effects of Extract of Grifola Frondosa on Bcl-2 and Bax in Spleen of Spleen Deficiency Mice

更新时间：2024-01-04

- Effects of Extract of Grifola Frondosa on Bcl-2 and Bax in Spleen of Spleen Deficiency Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 1, Pages: 182-184(2012)
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- Published：2012
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LEI Ping, MA Xian-de, GUAN Hong-quan. Effects of Extract of Grifola Frondosa on Bcl-2 and Bax in Spleen of Spleen Deficiency Mice[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 182-184.
DOI：

LEI Ping, MA Xian-de, GUAN Hong-quan. Effects of Extract of Grifola Frondosa on Bcl-2 and Bax in Spleen of Spleen Deficiency Mice[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 182-184. DOI：

摘要

目的 :探讨灰树花提取物对脾虚小鼠脾脏凋亡相关基因Bcl-2和Bax的影响。方法 :健康雄性清洁级昆明种小鼠48只随机分为6组

即空白组、脾虚组、阳性药组(香菇多糖

2 mg ·kg-1)、灰树花提取物低、中、高剂量组(5

20 mg ·kg-1)

每组8只。采用番泻叶-劳倦过度复合因素法造模14 d

之后灰树花提取物和香菇多糖溶液滤菌后ip 10 d

第11天处死动物。免疫组化法观察脾脏凋亡相关蛋白Bcl-2

Bax的表达

并用逆转录聚合酶链反应(RT-PCR)测定脾细胞凋亡相关基因Bcl-2

Bax mRNA的表达情况。结果 :给予灰树花提取物后

Bcl-2阳性产物平均灰度值比脾虚组明显降低(P<0.01)

Bax阳性产物平均灰度值比脾虚组明显升高(P<0.01)。给予灰树花提取物后

Bcl-2 mRNA表达显著升高(P<0.01)

Bax mRNA表达显著降低(P<0.01)。结论 :灰树花提取物可以通过促进Bc1-2蛋白与mRNA的表达、抑制Bax蛋白与mRNA的表达

提高Bcl-2/Bax的比值

可以通过影响线粒体途径来抑制脾淋巴细胞凋亡的发生。

Abstract

Objective :To investigate the effect of extract of grifola frondosa on Bcl-2 and Bax in spleen of spleen deficiency mouse. Method : Forty-eight KM mice were divided into 6 groups: normal group

spleen deficiency group

lentinan group(2 mg ·kg-1)

three groups of extract of grifola frondosa(5

20 mg ·kg-1)

ip ten days. Explore the expression of Bcl-2 and Bax in spleen of spleen deficiency mice by SP immunohistochemistry staining and RT-PCR. Result : The expression of Bcl-2 decreased

and the expression of Bax increased in spleen deficiency group. After given the extract of grifola frondosa

the expression of Bcl-2 increased significantly (P<0.01)

and the expression of Bax decreased significantly(P<0.01). Conclusion : The extract of grifola frondosa could maintain the immunologic function by upregulating the expression of Bcl-2 and downregulating the expression of Bax in spleen of spleen deficiency mice.

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Related Institution

Department of Pharmacy,Jiangsu University

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