Objective: To establish a HPLC method for the simultaneous determination of danshensu

tanshinone ⅡA and notoginsenoside R1

ginsenoside Rgl in Fufang Shengmai Granules. Method: Danshensu and tanshinone ⅡA were separated on the column of Discovery C18 (4.6 mm×250 mm

5 μm). The chromatographic condition for danshensu and tanshinone ⅡA was containing mobile phase

flow rate and detect wavelength.The gradient elution program was carried out by mobile phase of methanol(A)-0.5% acetic acid(B) that follow 0-10 min

9％-75% A; 10-40 min

75％ A.Flow rate was 1.0 mL·min-1 and temperature was 25 ℃. The analytes were detected at 275 nm.Chromatographic condition for notoginsenoside R1 and ginsenoside Rgl was acetonitrile -0.05% phosphoric acid(20 ∶80)as mobile phase. Flow rate was 1.0 mL·min-1 and temperature was 30 ℃. The analytes were detected at 203 nm. Result: Calibration curves of danshensu were linear from 0.099 6 to 1.195 2 μg(r=0.999 9)

the linearity range of tanshinoneⅡA was 0.026-0.312 μg(r=0.999 9)

the methodology recovery of danshensu and tanshinoneⅡA was 101.33%

100.24% respectively

RSD was 1.28%

1.53%; Calibration curves of notoginsenoside R1 were linear from 0.092 6 to 1.182 2 μg(r=0.999 7)

the linearity range of ginsenoside Rgl was 0.160 8-1.929 6 μg (r=0.999 9)

the methodology recovery of notoginsenoside R1 and ginsenoside Rgl was 99.87% and 101.42%

RSD was 1.07% and 2.43%. Conclusion: The HPLC method is simple and has satisfactory efficacy

it can simultaneously determine danshensu

tanshinone ⅡA and notoginsenoside R1

ginsenoside Rg1 from different areas.