Study for Determination of Related Substances in Salvianolic Acid A

YU Cui-cui; LIU Jun-feng; CHE Xin; LIU Ke

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Study for Determination of Related Substances in Salvianolic Acid A

更新时间：2024-01-04

- Study for Determination of Related Substances in Salvianolic Acid A
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 19, Pages: 94-97(2011)
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- Published：2011
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YU Cui-cui, LIU Jun-feng, CHE Xin, et al. Study for Determination of Related Substances in Salvianolic Acid A[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 94-97.
DOI：

YU Cui-cui, LIU Jun-feng, CHE Xin, et al. Study for Determination of Related Substances in Salvianolic Acid A[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 94-97. DOI：

摘要

目的: 建立一种测定丹酚酸A中有关物质含量的方法。方法: 用外标法测定丹酚酸C的含量

采用Diamonsil C18柱(4.6 mm×250 mm

5 μm)

乙腈-0.2%磷酸水溶液(30 ∶70)为流动相

流速1 mL·min-1

检测波长 285 nm

柱温 30 ℃

以主成分自身对照法控制其他杂质的总含量。结果: 丹酚酸C的含量均<1.0%

其他杂质峰面积和<3.0%。结论: 该方法专属性强

灵敏度高

简单准确

能有效控制丹酚酸A中有关物质的含量。

Abstract

Objective: To establish a method for the determination of reference substances in salvianolic acid A. Method: Diamonsil C18 column(4.6 mm×250 mm

5 μm)

acetonitrile-0.2% phosphoric acid solution(30 ∶70)as mobile phase

flow rate 1 mL·min-1

detection wavelength 285 nm

column temperature 30 ℃

other relative substances were controlled by main component self-compare method. Result: The content of salvianolic acid C was less than 1.0%

and the others were less than 3.0%. Conclusion: This method is specific

sensitive

simple

accurate

and can effectively detect salvianolic acid C.

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Related Institution

The First Clinical Medical College of Guangzhou University of Chinese Medicine

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