Effects of 6-gingerol on Transport Ability of PepT1 in Caco-2 Cells

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Effects of 6-gingerol on Transport Ability of PepT1 in Caco-2 Cells

更新时间：2024-01-04

- Effects of 6-gingerol on Transport Ability of PepT1 in Caco-2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 16, Pages: 116-119(2011)
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- Published：2011
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GUO Wen-feng, YANG Wei-peng, WANG Yi-wei, et al. Effects of 6-gingerol on Transport Ability of PepT1 in Caco-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 116-119.
DOI：

GUO Wen-feng, YANG Wei-peng, WANG Yi-wei, et al. Effects of 6-gingerol on Transport Ability of PepT1 in Caco-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 116-119. DOI：

摘要

目的: 观察6-姜酚对Caco-2细胞肽转运载体PepT1转运二肽的能力及其蛋白、mRNA表达的影响。方法: Caco-2细胞融合后连续培养28 d后给予6-姜酚处理

并设立正常对照组及cAMP抑制剂对照组

用放射性同位素示踪技术比较Caco-2细胞转运二肽化合物Glycyl-Sarcosine的能力

采用Western blot方法测定Caco-2细胞膜上PepT1蛋白的表达

荧光定量PCR方法测定PepT1 mRNA(SLC15A1)的表达水平。结果: 6-姜酚处理组Caco-2细胞60

120 min时点Glycyl-Sarcosine吸收转运累计量分别为(6.84±0.46)

(8.61±0.54)μmol/孔

高于正常对照组( P< 0.05)

后者分别为(5.62±0.20)、(6.54±0.54)μmol/孔;6-姜酚与Rp-8-Br-cAMP合用组120 min时点为(6.92±0.67)μmol/孔

明显低于相应时点单用6-姜酚组( P< 0.05);6-姜酚处理后Caco-2细胞膜PepT1蛋白表达增加

PepT1表达与内参GAPDH表达灰度比值6-姜酚组为(0.317±0.022)

高于空白对照组(0.220±0.019)( P< 0.01);荧光定量PCR结果

6-姜酚能上调Caco-2细胞SLC15A1 mRNA表达

但与cAMP抑制剂合用后该抑制作用被阻断。结论: 6-姜酚具有促进正常培养Caco-2细胞转运二肽化合物Glycyl-Sarcosine的作用

该作用可能与上调PepT1蛋白及mRNA表达有关

该过程调控与胞内第二信使cAMP有一定的关系。

Abstract

Objective: To observe the effects of 6-gingerol on expression and transport ability of PepT1 in Caco-2 cells. Method: Caco-2 monolayers were grown on permeable supports. Peptide transport activity was studied using -glycyl-sarcosine ( -Gly-Sar). The densities of PepT1 protein and mRNA (SLC15A1) expression levels were analyzed by Western blot and Real-time quantitative polymerase chain reaction. Result: The total transported Gly-Sar of Caco-2 cells within 60

120 minutes of group treated by 6-gingerol were higher than those of controlled group

(6.84±0.46)

(8.61±0.54) μmol/per well vs (5.62±0.20)

(6.54±0.54) μmol/per well

and higher than group of cells treated together with Rp-8-Br-cAMP within 120 minutes

the latter result were (6.92±0.67) μmol/per well. And 6-gingerol showed up-regulated effect on PepT1 protein and mRNA expression

also can be inhabited by Rp-8-Br-cAMP. Conclusion: 6-gingerol has significant effects on promoting the transport ability of dipeptides in Caco-2 cells

which maybe take effects by up-regulating PepT1 protein and mRNA expression.

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