Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 20, Pages: 92-94(2011)
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Published:2011
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DUAN Qiong, WANG Yang, LIN Ting-ting, et al. Determination of Dehydroeffusol in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 92-94.
DOI:
DUAN Qiong, WANG Yang, LIN Ting-ting, et al. Determination of Dehydroeffusol in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 92-94.DOI:
Objective: To establish a method of determined the content of dehydroeffusol in Juncus effusus . Method: The content of dehydroeffusol was determined by HPLC. The separation employed Agilent HC-C18 column (4.6 mm×250 mm
5 μm) in 23 ℃
using methanol-water (65 ∶35)as mobile phase at flow rate was 1.0 mL·min-1 and UV detection at 270 nm. Result: The method of validation parameters indicate a linear range of between 0.017 4-0.870 4 μg. The recovery was 98.3%(RSD 0.61%). Conclusion: The method is simple
accurate and sensitive. It is adoptable for quantitative analysis of dehydroeffusol in J. effusus.
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