HPLC Fingerprint Chromatogram of Yinhuang Series Preparations

LIU Yong-li; LI Dong-mei; FENG Li; YUAN Hao

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HPLC Fingerprint Chromatogram of Yinhuang Series Preparations

更新时间：2024-01-04

- HPLC Fingerprint Chromatogram of Yinhuang Series Preparations
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 20, Pages: 102-104(2011)
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- Published：2011
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LIU Yong-li, LI Dong-mei, FENG Li, et al. HPLC Fingerprint Chromatogram of Yinhuang Series Preparations[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 102-104.
DOI：

LIU Yong-li, LI Dong-mei, FENG Li, et al. HPLC Fingerprint Chromatogram of Yinhuang Series Preparations[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 102-104. DOI：

摘要

目的: 建立银黄系列品种的指纹图谱检测方法。方法: 采用C18色谱柱(4.6 mm×150 mm

5 μm)

流动相乙腈与0.1%磷酸溶液进行梯度洗脱

检测波长为318 nm。通过指纹图谱相似度评价软件

对银黄系列品种的指纹图谱进行了相似度计算。结果: 银黄胶囊、银黄含片、银黄颗粒、银黄口服液4个剂型指纹图谱整体一致

共有峰数量与比例稍有差别

各剂型10批样品相似度>0.9。结论: 所建立指纹图谱检测方法适用于银黄系列制剂的质量控制。

Abstract

Objective: To established the method of Yinhuang series preparations fingerprint chromatogram. Method: In this method

the C18 column (4.6 mm×150 mm

5 μm) was used

and acetonitrile-0.1% phosphoric acid was used as gradient eluent; detection wavelength was at 318 nm. Result: Yinhuang capsules

Yinhuang buccal tablets

Yinhuang granules and Yinhuang oral liquid were consistent with the overall fingerprint chromatogram

but the number and percentage of common peaks slightly different

10 batches similarity of Yinhuang series preparations are greater than 0.9. Conclusion: This method is useful for quality control of Yinhuang series preparations.

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Related Institution

College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine

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Hubei Institute for Food and Drug Control

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