Determination of Total Flavonoids in the Seeds of by HPLC

JU Cheng-guo; WANG Wei; ZHAO Huan-jun; LIU Wei

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Determination of Total Flavonoids in the Seeds of by HPLC

更新时间：2024-01-04

- Determination of Total Flavonoids in the Seeds of by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 1, Pages: 77-79(2012)
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- Published：2012
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JU Cheng-guo, WANG Wei, ZHAO Huan-jun, et al. Determination of Total Flavonoids in the Seeds of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 77-79.
DOI：

JU Cheng-guo, WANG Wei, ZHAO Huan-jun, et al. Determination of Total Flavonoids in the Seeds of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(1): 77-79. DOI：

摘要

目的: 建立HPLC测定急性子中总黄酮含量的方法。方法: 采用Eclipse XDB-C18柱(4.6 mm×150 mm

5 μm);流动相甲醇-0.4%磷酸水(55 ∶45)

检测波长365 nm

柱温30 ℃

流速1.0 mL ·min-1。结果: 槲皮素、山柰酚、异鼠李素的线性范围分别为 0.111 2~0.556 0(r=0.999 4)

0.007 711~0.023 23(r=0.999 4)

0.006 69~0.033 45 μg (r=0.999 2);平均加样回收率分别为98.5% (RSD 1.3%

n=6)

97.3% (RSD 1.7%

n=6)

97.6% (RSD 1.6%

n=6)。结论: 该方法准确度高

重复性好

可用于急性子中总黄酮的含量测定。

Abstract

Objective: To develop a HPLC method for the determination of total flavonoids in the seeds of Impatiens balsamina. Method : An Eclipse XDB-C18 column (4.6 mm×150 mm

5 μm) was used at 30 ℃ with the mobile phase of methanol-0.4% phosphoric acid (55 ∶45). The detection wavelength was 365 nm and the flow rate wsa set at 1.0 mL ·min-1. Result :The linear response range for quercetin

kaempferol and isorhamnetin were 0.111 2-0.556 0 μg (r=0.999 4)

0.007 711-0.023 23 μg (r=0.999 4) and 0.006 69-0.033 45μg (r=0.999 2) respectively. The average recoveries were 98.5% (RSD 1.3%

n=6)

97.3% (RSD 1.7%

n=6) and 97.6% (RSD 1.6%

n=6). Conclusion : The method could be used for determination of total flavonoids of the seeds of I. balsamina.

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