Comparison of Liquiritin Content in Different Methods of Sini Decoction

YANG Yu-qin; LIANG Guang-yi; QIN Li-fen; LIU Jin-huan; HE Zhu-ying; CAO Pei-xue

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Comparison of Liquiritin Content in Different Methods of Sini Decoction

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- Comparison of Liquiritin Content in Different Methods of Sini Decoction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 24, Pages: 40-43(2011)
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- Published：2011
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YANG Yu-qin, LIANG Guang-yi, QIN Li-fen, et al. Comparison of Liquiritin Content in Different Methods of Sini Decoction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 40-43.
DOI：

YANG Yu-qin, LIANG Guang-yi, QIN Li-fen, et al. Comparison of Liquiritin Content in Different Methods of Sini Decoction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(24): 40-43. DOI：

摘要

目的: 对四逆汤传统汤剂、经方颗粒剂及单味配方颗粒剂中甘草苷的含量进行比较。方法: 采用高效液相色谱法

Diamonsil C18色谱柱(4.6 mm×150 mm

5 μm)

流动相乙腈-0.5%冰乙酸(20 ∶80)

流速0.7 mL·min-1

检测波长276 nm

柱温25 ℃

测定3种剂型中甘草苷的含量。结果: 甘草苷(每处方)在传统汤剂中含量为31.13 mg

经方颗粒剂中含量为48.48 mg

单味配方颗粒剂中含量为31.95。结论: 3种剂型中指标性成分甘草苷的含量有显著性差异。可为四逆汤剂型的进一步研究提供科学依据。

Abstract

Objective: To compare the content of liquiritin in Sini traditional decoction

the granules and the ingle-flavor granules. Method :To determine the content of liquiritin by HPLC method Diamonsil C18 column (4.6 mm×150 mm

5 μm)was

used

the mobile phase of acetonitrile-0.1% acetic acid-water(20 ∶80). The flonrate was 0.7 mL·min-1 and the detection wave length was at 276 nm. The tem perature of colcomn was 25 ℃. Result :The content of liquiritin was 31.13 mg in traditional decoction

48.48 mg in the granules and 31.95 mg in the ingle-flavor granules in a prescription. Conclusion :The content of liquiritin is significantly different formulations

which can provide the scientific basis for further research of Sini decoction type.

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