Objective: To provide scientific experimental basis and reliable methods for establishing the quality standards and exploiting resources of the traditional Chinese medicine Gekko swinhonis. Method: To compare the habitat and organ difference of protein

the water-soluble protein of different parts (body and tail) of G. swinhonis from different habitats was extracted. The protein contents were determined and the experiments of PAGE were conducted. Result: The protein content of G. swinhonis varied with the habitat

and it also varied with the part from the same habitat. The PAGE electrophoretogram showed that except for the common bands

there was difference in the distribution and number of the protein bands among different samples. Conclusion: The method of coomassie brilliant blue staining is simple and fast with good correlation coefficient

and can be used to determine the protein content of medicinal materials. The experiment of PAGE is rapid

accurate and reproducible

and the protein electrophoresis fingerprint can serve as one of the important bases for the quality evaluation and resources exploitation of G. swinhonis.