Extraction Process of Flavone in  Based on Cellulase

LAN Jun-feng; XIE Ji-yun; YU Lan

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Extraction Process of Flavone in Based on Cellulase

更新时间：2024-01-04

- Extraction Process of Flavone in Based on Cellulase
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 16, Pages: 26-28(2011)
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- Published：2011
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LAN Jun-feng, XIE Ji-yun, YU Lan. Extraction Process of Flavone in Based on Cellulase[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 26-28.
DOI：

LAN Jun-feng, XIE Ji-yun, YU Lan. Extraction Process of Flavone in Based on Cellulase[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 26-28. DOI：

摘要

目的: 优选纤维素酶辅助提取叶下珠黄酮提取工艺条件

为叶下珠的高效利用提供依据。方法: 采用单因素试验研究料液比、提取温度、提取时间和酶浓度对叶下珠黄酮提取得率的影响

通过正交试验优选纤维素酶辅助提取叶下珠黄酮的工艺条件。结果: 纤维素酶辅助提取叶下珠黄酮的最佳工艺条件为酶质量浓度4.25 g·L-1

提取温度40 ℃

料液比1∶30

提取时间1.5 h。在此条件下

黄酮的提取得率为1.51%。结论: 采用纤维素酶辅助提取叶下珠黄酮

提取效果较好

该方法具有较好的应用前景。

Abstract

Objective: To optimize the extraction process of flavone in Phyllanthus urinaria based on celluase

and provide the basis for the efficient use of P. urinaria. Method: The single factor test was performed to study the effects of the material-liquid ratio

extraction temperature

extraction time and the concentration of cellulase on the yield rate of flavone from P.urinaria. The process of extracting flavone based on cellulose was optimized with orthogonal test. Result: The results showed that the optimized extraction condition of flavone in P.urinaria based on cellulase was as following: the concentration of celluase was 4.25 g·L-1

the extraction temperature was 40 ℃

the material-liquid ratio was 1∶30 and the extraction time was 1.5 h. Under the optimized condition

the extraction ratio of flavone was high of 1.51%. Conclusion: In the present conditions

the extracting efficiency of flavone from P.urinaria based on celluase is better

and it has a promising future in application.

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Related Author

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NI Ning

WANG Yu-rong

WU Hua

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CHEN Bi-qiong

ZHANG Yan-jun

LAN Jun-feng

Related Institution

School of Chemistry and Chemical Engineering,Guangxi University

Department of Chemistry and Life Science,Liuzhou Teachers College

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Medical School， Lijiang Culture and Tourism College

Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Ministry of Education，Jiangxi University of Chinese Medicine

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