Objective: To investigate the effect of buddlejasaponin Ⅳ on proliferation and activation of rat hepatic stellate cell line HSC-T6. Method: HSC-T6 cells was incubated with buddlejasaponin Ⅳ at different concentrations (20

14

9.8

6.86 mg·L-1) for 24 hours.Effect of buddlejasaponin Ⅳ on proliferation of HSC-T6 was observed by MTT assay.Col-I and Col-Ⅲ mRNA expressions were assayed by reverse transcription ploymerase chain reaction (RT-PCR) after incubation.Apoptosis of HSC-T6 was detected by Annexin V/ PI staining combined with flow cytometry method. Result: Buddlejasaponin Ⅳ could inhibit HSC-T6 cell proliferation

in a dose-and time-dependent manner.Buddlejasaponin Ⅳ with 6.86 mg·L-1 inhibited Col-Ⅲ mRNA expression(P<0.05).Buddlejasaponin Ⅳ with 9.8 mg·L-1 decreased Hyp secretion

inhibited Col-I mRNA expression (P<0.05) and induced apoptosis significantly (P<0.01)

so as to achieve the effect of anti-hepatic fibrosis. Conclusion: Buddlejasaponin Ⅳcould interfere liver fibrosis via inhibiting proliferation and activation of HSC-T6 cells and inhibiting the synthesis of extracellular matrix.