Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin
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Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 17, Pages: 13-16(2011)
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Published:2011
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XIA Lin-bo, LI Bo, DENG Shi-ren, et al. Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 13-16.
DOI:
XIA Lin-bo, LI Bo, DENG Shi-ren, et al. Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 13-16.DOI:
Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin
Objective: To investigate the purification method of two main flavonoid aglycones (apigenin and genkwanin) from Flos Genkwa by macroporous resin. Method: HPLC was used to determine the content of apigenin and genkwanin
and the purification conditions were selected by static and dynamic adsorption. Result: AB-8 resin was selected because it gave the most effective of separation. The optimum process for the purification was as follows: the concentration of sample solution was 0.1 g ·mL-1
the dosage was 5 BV
adsorption flow rate was 2 BV ·h-1
and eluted with 95% ethanol by 9 BV
elution flow rate was 2 BV ·h-1. The purity of two flavonoid aglycones was more than 20% after processing. Conclusion: Apigenin and genkwanin were purified effectively with macroporous resin. The optimum process is simple