Separation and Purification process of Total Alkaloids from Sinisan
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Separation and Purification process of Total Alkaloids from Sinisan
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 17, Pages: 11-13(2011)
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Published:2011
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SUN Zhi-ming, DUAN Zhi-gang, JIAO Liang, et al. Separation and Purification process of Total Alkaloids from Sinisan[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 11-13.
DOI:
SUN Zhi-ming, DUAN Zhi-gang, JIAO Liang, et al. Separation and Purification process of Total Alkaloids from Sinisan[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 11-13.DOI:
Separation and Purification process of Total Alkaloids from Sinisan
Objective: To study the purification technological parameters of total alkaloids from Sinisan by 001×7 cation resin. Method: Eluting solvent
sample concentration
absorption capacity and the volume of eluting solvent were chosen to analyze their influence on the purification process. UV was used to determine the process parameters by compared with synephrine
and the content of total alkaloids from eluent as criteria. Result: Eluting solvent was 4% ammonia ethanol solution
the optimum sample concentration was 0.25 g ·mL-1
the absorption capacity is 5 mL and the volume of eluting solvent is eight times to the volume of sample solution. Conclusion: Total alkaloids from Sinisan can be purified by cation resins.The purification process is simple and affordable.