Differerntiation of Mesenchymal Stem Cells into Myofibroblasts and the Impact of Yiguanjian

YAN Xiao-feng; YE Ting-jie; LIU Hui-yang; WANG Xiao-ling

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Differerntiation of Mesenchymal Stem Cells into Myofibroblasts and the Impact of Yiguanjian

更新时间：2024-01-04

- Differerntiation of Mesenchymal Stem Cells into Myofibroblasts and the Impact of Yiguanjian
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 22, Pages: 123-126(2011)
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- Published：2011
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YAN Xiao-feng, YE Ting-jie, LIU Hui-yang, et al. Differerntiation of Mesenchymal Stem Cells into Myofibroblasts and the Impact of Yiguanjian[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 123-126.
DOI：

YAN Xiao-feng, YE Ting-jie, LIU Hui-yang, et al. Differerntiation of Mesenchymal Stem Cells into Myofibroblasts and the Impact of Yiguanjian[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 123-126. DOI：

摘要

目的: 观察体外诱导骨髓间充质干细胞向肌成纤维细胞分化及一贯煎的影响。方法: 骨髓间充质干细胞分离、培养和鉴定

取第3代细胞加入转化生长因子(TGF-β1 5 μg·L-1)诱导定向分化。骨髓间充质干细胞随机分为正常组、TGF-β1诱导组

TGF-β1诱导加一贯煎组

2周后通过免疫荧光、实时定量PCR法检测骨髓间充质干细胞分化情况及一贯煎的影响。结果: 骨髓间充质干细胞鉴定结果显示CD44强阳性表达

CD29弱阳性表达。TGF-β1诱导后

免疫荧光及实时定量PCR均显示TGF-β1诱导组细胞α-SMA阳性表达

一贯煎组细胞α-SMA表达显著降低

差异有统计学意义(P<0.05)。结论: 经体外诱导骨髓间充质干细胞可以向肌成纤维细胞分化

一贯煎可阻止骨髓间充质干细胞向肌成纤维细胞分化。

Abstract

Objective: To observe the differentiation of bone marrow mesenchymal stem cells into myofibroblast in vitro and the impact of Yiguanjian. Method: Bone marrow mesenchymal stem cells were separated

cultured and identified

transforming growth factor β1 (TGF-β1 5 μg·L-1) was added to the third-generation cells to induce differentiation. Bone marrow mesenchymal stem cells were divided into normal group

TGF-β1 induced group

TGF-β1 induced plus Yiguanjian group. 2 weeks later

bone marrow mesenchymal stem cells were harvested and detected using immunofluorescence and real-time quantitative PCR. Result: The result of bone marrow mesenchymal stem cells’ identification showed the strong expression of CD44 and the weak expression of CD29. After TGF-β1 induced

the results of immunofluorescence and real-time quantitative PCR showed that the group of TGF-β1 induced the expression of smooth muscle ectin(α-SMA)

the expression of α-SMA in the group of TGF-β1 induced plus Yiguanjian was significantly reduced

the difference was statistically significant (P <0.05). Conclusion: Bone marrow mesenchymal stem cells can differentiate into myofibroblasts using TGF-β1 induction

Yiguanjian can prevent the bone marrow mesenchymal stem cells’ differerntiation to myofibroblast.

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