Protective Effects of Chlorogenic Acid on Liver Injury in Mice

SHI Xiu-ling; GAO Yin-hui

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Protective Effects of Chlorogenic Acid on Liver Injury in Mice

更新时间：2024-01-04

- Protective Effects of Chlorogenic Acid on Liver Injury in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 19, Pages: 199-202(2011)
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- Published：2011
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SHI Xiu-ling, GAO Yin-hui. Protective Effects of Chlorogenic Acid on Liver Injury in Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 199-202.
DOI：

SHI Xiu-ling, GAO Yin-hui. Protective Effects of Chlorogenic Acid on Liver Injury in Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(19): 199-202. DOI：

摘要

目的: 研究绿原酸对四氯化碳(CCl4)所致肝损伤小鼠的保护作用。方法: 将60只昆明种小鼠随机分为6组

各组每天ig给药容量为20 mL·kg-1·d-1

连续10 d。正常组和模型组给予蒸馏水

阳性组给予联苯双酯0.60 g·kg-1

绿原酸高、中、低剂量组分别给予绿原酸14

3.5 mg·kg-1。末次给药后禁食不禁水

8 h后

除正常组外

其余各组均ip 0.08% CCl4花生油溶液10 mL·kg-1

12 h后眼球取血

分离血清

检测血清中的谷丙转氨酶(ALT)和谷草转氨酶(AST)

同时测定肝匀浆中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)的水平。将肝大叶HE染色

观察绿原酸对小鼠肝脏组织病理学的影响。结果: 绿原酸14

3.5 mg·kg-1剂量组小鼠的肝指数依次为(2.00±1.16)

(2.40±1.52)

(3.20±1.09)%

脾指数为(0.29±2.61)

(0.36±0.19)

(0.49±1.21)%;小鼠血清中ALT

AST活性依次分别为(40.01±7.14)

(51.19±9.45)

(62.66±9.01)U·L-1

(43.59±4.32)

(52.99±5.97)

(66.97±7.34)U·L-1

小鼠肝组织中MDA 的水平依次为(21.68±10.28)

(28.54±6.84)

(35.51±9.87) nmol·g-1

均明显低于模型组

差异有统计学意义(P<0.01)。绿原酸14

3.5 mg·kg-1剂量组

小鼠肝组织中SOD活性依次为(112.98±8.41)

(98.36±11.18)

(81.76±9.88) U·g-1

GSH-Px活性依次为(196.19±10.56)

(169.77±13.61)

(141.57±15.11) U·g-1

均明显高于模型组

差异有统计学意义(P<0.01)。同时

绿原酸各剂量组肝组织病变程度明显减轻

肝细胞再生明显

偶伴有点状坏死

汇管区少量炎细胞浸润。结论: 绿原酸具有显著的抗肝损伤的作用

其作用可能与其能清除体内自由基和抗氧化的作用有关。

Abstract

Objective: To observe the protect effects of chlorogenic acid on liver-injury of mice induced by Carbon Tetrachlotide (CCl4). Methods: Animals were devided into 6 groups:normal group

model group

solvent group

the chlorogenic acid high-dose group

middle-dose group

low-dose group. The dose of chlorogenic acid is ig 14

3.5 g·kg-1respectively. The mice models of acute chemical liver injury was induced by CCl4 . The indexes of the live and spleen

alanine transaminase(ALT) and aspartate transaminase(AST) activity in serum were examined. Superoxide dismutase(SOD)

glutathione peroxidase(GSH-Px)activity

MDA level in liver tissue were determined by immunological liver injury. Meanwhile liver pathological examination was observed by light microscope. Result: The chlorogenic acid (14

3.5mg·kg-1)could remarkablly resist the increase of ALT(40.01±7.14)

(51.19±9.45)

(62.66±9.01)U·L-1

AST (43.59±4.32)

(52.99±5.97)

(66.97±7.34)U·L-1

decreased the MDA level (21.68±10.28)

(28.54±6.84)

(35.51±9.87) nmol·g-1

and could improve SOD activity (112.98±8.41)

(98.36±11.18)

(81.76±9.88) U·g-1and GSH-Px level (196.19±10.56)

(169.77±13.61)

(141.57±15.11) U·g-1 (P<0.01 versus model group for above results). Conclusion: Chlorogenic acid has remarkable protective effects on liver –injury in mice.

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