Objective: To establish the method for the content determination of paeoniflorin and salvianolic acid B in Huoluoxiaoling granules. Method: HPLC was adopted to determine the content of paeoniflorin and salvianolic acid B. The column for paeoniflorin is Zorbax SB-C18(4.6 mm×150 mm

5 μm). The mobile phase is consisted of acetonitrile-water(10 ∶90)with the detection wavelength at 230 nm. The column temperature was at 40 ℃ and the flow rate was set at 1.0 mL·min-1. The column for salvianolic acid B is Zorbax Extend-C18(4.6 mm×150 mm

5 μm). The mobile phase is consisted of methanol-acetonitrile-0.1% formic acid(22 ∶10 ∶68)with the detection wavelength at 286 nm. The column temperature was at 25 ℃ and the flow rate was set at 1.0 mL·min-1. Result: There was good linearity between the peak area and concentration within the range of 0.084 4 -0.928 4 μg(r=0.999 8)for paeoniflorin and 0.226-2.034 μg(r=0.999 9)for salvianolic acid B respectively. The average recoveries of paeoniflorin and salvianolic acid B were 100.4%(RSD 2.30%)and 100.7%(RSD 2.73%)respectively( n ＝6). Conclusion: The method is simple

accurate and specific for quality control of Huoluoxiaoling granules.